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DSMZ

DSMZ 503e . THERMODESULFITIMONAS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 503e #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 962 ml - -
Solution B 20 ml - -
Solution C 1 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
1 Sparge solution A with 100% CO2 gas for 30 – 45 min to make it anoxic, distribute under the same gas atmosphere into anoxic serum vials to 20% of their volume, and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C, D, and E are prepared under 100% N2 gas and sterilized by filtration. To complete the medium, appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Adjust the pH of the complete medium to 6.0 - 6.5, if necessary.
2 The addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% (v/v) inoculum.
3 After inoculation add sterile H2 gas to 1 bar overpressure.

Solution A #

962
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.208 1.528
NH4Cl 0.25 g 0.26 4.858
NaCl 1.00 g 1.04 17.788
MgCl2 x 6 H2O 0.40 g 0.416 2.045
KCl 0.50 g 0.52 6.972
CaCl2 x 2 H2O 0.15 g 0.156 1.061
Wolfe's mineral elixir 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.2e-4 0.002
Distilled water 960.00 ml - -

Solution B #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO3 0.6 g 60 476.002
Distilled water 10.0 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferrous chloride solution (5.2%) 10 ml - -

Wolfe's mineral elixir #

1000
Solution occurs in 22 other media (first defined in Medium 792).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x H2O 5.00 g 5 29.583
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 0.379
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 307 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Ferrous chloride solution (5.2%) #

10
Solution occurs in 1 other media (first defined in Medium 916).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 0.52 g 52 261.554
Distilled water 10.00 ml - -
1 Adjust to pH 2 with HCl.

Metadata

Last modified: 18.11.25
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0 - 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 50 % CO2
  • 50 % H2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 0.997 9.407
Na2SO3 0.598 4.746
FeCl2 x 4 H2O 0.518 2.608
KCl 0.499 6.687
MgCl2 x 6 H2O 0.399 1.962
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
nutrition chemolithoautotroph 1 strain
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