Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.0 | g | 1 | 18.695 | |
K2HPO4 | 0.3 | g | 0.3 | 1.722 | |
KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
MgCl2 x 6 H2O | 20.0 | g | 20 | 98.375 | |
NaCl | 100.0 | g | 100 | 1711.16 | |
CaCl2 x 2 H2O | 2.7 | g | 2.7 | 18.366 | |
KCl | 4.0 | g | 4 | 53.655 | |
Na2SO4 | 3.0 | g | 3 | 21.121 | |
Na-acetate | 1.0 | g | 1 | 12.19 | |
Trypticase peptone
(BD BBL) |
1.0 | g | 1 | - | |
Yeast extract
(OXOID) |
1.0 | g | 1 | - | |
Na-(L)-lactate | 2.5 | g | 2.5 | - | |
Trace element solution SL-10 | 1.0 | ml | - | - | |
Selenite-tungstate solution | 1.0 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
Na2S x 9 H2O | 0.3 | g | 0.3 | 1.249 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve ingredients except sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 6.8 - 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 7.0, if necessary. A small amount of white precipitate forms after autoclaving, but this has no negative effect on growth. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.5 | g | 0.5 | 12.501 |
Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 28.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.0 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 100 | 1711.16 |
MgCl2 x 6 H2O | 20 | 98.375 |
KCl | 4 | 53.655 |
Na2SO4 | 3 | 21.121 |
CaCl2 x 2 H2O | 2.7 | 18.366 |
Show more |