482
482 .
ANAEROBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 482 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.33 | g | 0.33 | 2.425 | |
| NH4Cl | 0.33 | g | 0.33 | 6.169 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.33 | 1.623 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.33 | 2.245 | |
| KCl | 0.33 | g | 0.33 | 4.427 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| NaHCO3 | 1.50 | g | 1.5 | 17.856 | |
| Sucrose | 2.00 | g | 2 | 5.843 | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.3 | 1.708 | |
| Na2S x 9 H2O | 0.30 | g | 0.3 | 1.249 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, sucrose, cysteine and sulfide), adjust pH to 7.0, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to pH 7.0, if necessary. | |||||
Solution occurs in 265
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 28.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sucrose | 2 | 5.843 |
| NaHCO3 | 1.5 | 17.856 |
| Yeast extract | 1 | - |
| NH4Cl | 0.33 | 6.169 |
| KCl | 0.33 | 4.427 |
| Show more | ||
