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DSMZ

DSMZ 480b . TTD-MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Thermococcus stetteri DSM 5262

This strain uses the standard medium without modifications.

Growth conditions: 75 °C

Main sol. 480b #

ml
Equipment needed: Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.33 6.169
CaCl2 x 2 H2O 0.33 g 0.33 2.245
MgCl2 x 6 H2O 0.33 g 0.33 1.623
KCl 0.33 g 0.33 4.427
KH2PO4 0.33 g 0.33 2.425
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Sulfur
(powder) 		10.00 g 10 311.818
Distilled water 1000.00 ml - -
1 Add to the medium 0.5% casitone and 2.5% NaCl (marine source). Adjust pH to 5.7 with hydrochloric acid before sterilization. Sterilize the medium by heating for 1 h at 90 - 100°C on three successive days. Before use add 30.0 ml/l of 5% (w/v) sterile, anaerobic NaHCO3-solution, vitamins and sulfide. Final pH should be 6.5.
Casitone 0.50 % 5 -
NaCl 2.50 % 25 427.789
NaHCO3
(5% w/v) 		30.00 ml 1.5 17.856
Wolin's vitamin solution 10.00 ml - -
Na2S x 9 H2O 0.50 g 0.5 2.082

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution #

1000
Solution occurs in 54 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 0.021
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Filter, Hungate tubes
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Associated strains

All strains for this medium: 1
Thermococcus stetteri DSM 5262

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
Sulfur 10 311.818
Casitone 5 -
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.5 2.082
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain