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DSMZ

DSMZ 463a . EDTA-MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Stenotrophomonas chelatiphaga DSM 21508

* Modification: Reactivate freeze-dried cells from ampoules in liquid medium 1

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C

Main sol. 463a #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.41 g 0.41 2.888
KH2PO4 0.26 g 0.26 1.911
MgSO4 x 7 H2O 1.00 g 1 4.057
CaCl2 x 2 H2O 0.40 g 0.4 2.721
Trace element solution 1.00 ml - -
Vitamin solution 1.00 ml - -
EDTA
(di- or trisodium salt) 		0.50 g 0.5 1.711
Distilled water 1000.00 ml - -
1 pH 8.0

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium 463).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 68.0 mg 0.068 0.499
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 120.0 mg 0.12 0.924
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 24.0 mg 0.024 0.099
HCl , 0.05 molar 1000.0 ml - -

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Stenotrophomonas chelatiphaga DSM 21508

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1 4.057
EDTA 0.5 1.711
Na2HPO4 x 2 H2O 0.41 2.888
CaCl2 x 2 H2O 0.4 2.721
KH2PO4 0.26 1.911
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
phyla 1 2 strains
oxygen aerobe 2 strains
oxygen obligate aerobe 2 strains
temperature mesophilic 2 strains