456
456 .
KPL MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 456 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Lactic acid whey | 1000 | ml | - | - | |
| White table wine | 140 | ml | - | - | |
| Glucose | 10 | g | 8.764 | 48.647 | |
| Galactose | 10 | g | 8.764 | 48.647 | |
| Tween 80 | 1 | ml | - | - | |
| Agar | 15 | g | 13.146 | 39.088 | |
| 1 Adjust pH to 5.5 | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Skim milk | 10 | % | 100 | - | |
| 1 Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. | |||||
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| White wine | 200 | ml | - | - | |
| 1 Filter-sterilize and add to autoclaved basal medium. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.5 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Skim milk | 87.642 | - |
| Agar | 13.146 | 39.088 |
| Galactose | 8.764 | 48.647 |
| Glucose | 8.764 | 48.647 |
| White wine | - | - |
| Show more | ||
