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DSMZ

DSMZ 452 . GLUCOSE MEDIUM (NAKAYAMA)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Sporolactobacillus laevolacticus DSM 6547

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 452 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 10 g 10 55.506
Peptone 10 g 10 -
Yeast extract 15 g 15 -
Agar 10 g 10 29.733
Distilled water 980 ml - -
Solution A 10 ml - -
Solution B 10 ml - -
Solution C 1 ml - -
1 Autoclave all solutions separately, final pH is 6.8.

Solution A #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.5 g 5 36.742
K2HPO4 0.5 g 5 28.707
Distilled water 100.0 ml - -

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 3.00 g 30 121.716
NaCl 0.10 g 1 17.112
MnSO4 x 5 H2O 0.10 g 1 4.148
CuSO4 x 5 H2O 0.01 g 0.1 0.401
Distilled water 100.00 ml - -

Solution C #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
Na3-citrate 2.0 g 20 77.499
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 14
Sporolactobacillus laevolacticus DSM 6547

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 15 -
Glucose 10 55.506
Agar 10 29.733
Peptone 10 -
MgSO4 x 7 H2O 0.3 1.217
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Cultivation metadata from 14 strains BacDive

phyla 1 14 strains
temperature mesophilic 14 strains