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Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 953 | ml | - | - | |
Solution B | 50 | ml | - | - | |
Standard vitamin solution | 5 | ml | - | - | |
1 Sterilize solution A separately at 121°C for 15 min., cool to 50°C and then mix aseptically with filter-sterilized solution B and 5.0 ml of filter-sterilized standard vitamin solution (see medium 428). | |||||
2 RBA is an ammonium-free medium which has successfully been used for the isolation, growth and purity check of a broad spectrum of nitrogen fixing bacteria (Ref. 3363). For microaerophilic nitrogen-fixing bacteria use semisolid medium with 0.3% end concentration of agar and incubate the liquid cultures under 10% (v/v) air and 90% (v/v) N2. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.10 | g | 0.105 | 0.771 | |
K2HPO4 | 0.90 | g | 0.944 | 5.422 | |
NaCl | 0.10 | g | 0.105 | 1.796 | |
CaCl2 x 2 H2O | 0.10 | g | 0.105 | 0.714 | |
MgSO4 x 7 H2O | 0.10 | g | 0.105 | 0.426 | |
Na2MoO4 x 2 H2O | 5.00 | mg | 0.005 | 0.022 | |
NaVO3 x H2O | 5.00 | mg | 0.005 | 0.037 | |
MnSO4 x H2O | 5.00 | mg | 0.005 | 0.031 | |
FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.038 | |
Yeast extract | 0.05 | g | 0.052 | - | |
Trace element solution SL-6 | 3.00 | ml | - | - | |
Distilled water | 950.00 | ml | - | - | |
Agar , if required | 15.00 | g | 15.74 | 46.799 | |
1 Adjust pH to 7.3. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Disodium succinate | 1 | g | 20 | 123.419 | |
DL-Malate | 2 | g | 40 | 298.307 | |
Na-pyruvate | 1 | g | 20 | 181.745 | |
D-Mannitol | 2 | g | 40 | 219.575 | |
D-Glucose | 2 | g | 40 | 222.025 | |
Distilled water | 50 | ml | - | - | |
1 Adjust pH to 7.3. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
H3BO3 | 0.30 | g | 0.3 | 4.851 |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Riboflavin | 10.0 | mg | 0.1 | 0.266 | |
Thiamine-HCl x 2 H2O | 50.0 | mg | 0.5 | 1.339 | |
Nicotinic acid | 50.0 | mg | 0.5 | 4.061 | |
Pyridoxine hydrochloride | 50.0 | mg | 0.5 | 2.431 | |
Calcium pantothenate | 50.0 | mg | 0.5 | 1.049 | |
Biotin | 0.1 | mg | 1.0e-3 | 0.004 | |
Folic acid | 0.2 | mg | 0.002 | 0.005 | |
Vitamin B12 | 1.0 | mg | 0.01 | 0.007 | |
Distilled water | 100.0 | ml | - | - | |
1 Filter sterilize (0.2 µm pore-size) and store at 4°C. |
Last modified: | 20.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 14.881 | 44.245 |
DL-Malate | 1.984 | 14.797 |
D-Glucose | 1.984 | 11.013 |
D-Mannitol | 1.984 | 10.892 |
Na-pyruvate | 0.992 | 9.015 |
Show more |
phyla | 2 | 49 strains |
oxygen | obligate aerobe | 1 strain |
temperature | mesophilic | 49 strains |