438
438 .
BORDET-GENGOU-MEDIUM (DIFCO)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Bordetella pertussis DSM 4925
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 438 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bordet-Gengou-Agar-Base | 1000 | ml | - | - | |
| Horse blood | 15 | % | 150 | - | |
| 1 Bordet-Gengou-Agar-Base supplemented with 15% horse blood. | |||||
| 2 Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 1. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , for solid medium | 15 | g | 15 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Solution occurs in 22
other media (first defined in Medium 220).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 15 | g | - | - | |
| Soy peptone | 5 | g | - | - | |
| NaCl | 5 | g | - | - | |
| Agar | 15 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||
Metadata
| Last modified: | 19.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 9 |
Bordetella pertussis DSM 4925
No modifications necessary for this strain.
Media similar to medium 438
| 429 | COLUMBIA BLOOD AGAR | |
| 429e | e. COLUMBIA BLOOD AGAR 10% | |
| 420 | BLOOD AGAR I |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Horse blood | 150 | - |
| Bordet-Gengou-Agar-Base | - | - |
