429 .
COLUMBIA BLOOD AGAR
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* Medium for Bartonella elizabethae DSM 28217
(with 10% horse blood); plus 5% CO2
Equipment needed:
Final gas composition:
5 % CO2
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Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Columbia agar base | 1000 | ml | ||
|
|
Horse blood | 4 | % | ||
|
|
Horse blood | 100 | g/l | ||
| 1 Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). | |||||
545. TRYPTONE SOYA BROTH (TSB) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D(+)-Glucose | 2.5 | g | ||
|
|
NaCl | 5.0 | g | ||
|
|
K2HPO4 | 2.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
215. BHI MEDIUM #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Brain heart infusion
(Difco) |
37 | g |
|
|
Distilled water | 1000 | ml |
1. NUTRIENT BROTH #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||