429
429 .
COLUMBIA BLOOD AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Simonsiella muelleri DSM 2579
* Modification: Add enough liquid medium 220, 535 or 215 to the agar plate when inoculating. The surfaces of agar plates must be moist during the whole incubation period
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 37 °C
Main sol. 429 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia agar base | 1000 | ml | - | - | |
| Horse blood | 4 | % | 40 | - | |
| 1 Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). | |||||
Solution occurs in 4
other media (first defined in Medium 54a).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
Solution occurs in 2
other media (first defined in Medium 429).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Brain heart infusion
(Difco) |
37 | g | 37 | - |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 429).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
Metadata
| Last modified: | 20.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Media similar to medium 429
| 438 | BORDET-GENGOU-MEDIUM (DIFCO) | |
| 429e | e. COLUMBIA BLOOD AGAR 10% | |
| 420 | BLOOD AGAR I |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Horse blood | 40 | - |
| Columbia agar base | - | - |
Cultivation metadata from 116 strains
| halophily | halotolerant | 1 strain |
| pH | 6 - 8 | 4 strains |
| phyla | 4 | 117 strains |
| nutrition | chemoorganotroph | 2 strains |
| oxygen | microaerophile | 44 strains |
| Show more | ||