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CLOSTRIDIUM ACETOBUTYLICUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium sp. DSM 6646
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Main sol. 411 #
ml
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Potato
(fresh, washed, peeled and sliced) |
200.0 | g | 200 | - | |
| CaCO3 | 2.0 | g | 2 | 19.982 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| D-Glucose | 6.0 | g | 6 | 33.304 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix potatoes and CaCO3 then autoclave 30 min at 121°C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Adjust pH of complete medium to 6.8. | |||||
| 2 Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium: | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Milk
(fresh) |
100.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.1 | ml | 1.0e-4 | - | |
| 1 Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121°C. | |||||
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 17 |
Clostridium sp. DSM 6646
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Potato | 200 | - |
| D-Glucose | 6 | 33.304 |
| CaCO3 | 2 | 19.982 |
| L-Cysteine HCl x H2O | 0.5 | 2.847 |
| Sodium resazurin | 5.0e-4 | - |
| Show more | ||
Cultivation metadata from 17 strains
| phyla | 1 | 17 strains |
| oxygen | anaerobe | 17 strains |
| temperature | mesophilic | 17 strains |