405
405 .
SP2 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 405 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casitone
(Difco) |
1.00 | g | 1 | - | |
|
Yeast extract
(Difco) |
0.20 | g | 0.2 | - | |
| Na-acetate | 0.02 | g | 0.02 | 0.244 | |
|
Agar
, if required
(Difco) |
15.00 | g | 15 | 44.599 | |
| Artificial sea water OR Sea water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 If natural sea water is used, the pH need not be adjusted. | |||||
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 24.7 | g | 24.7 | 422.656 | |
| KCl | 0.7 | g | 0.7 | 9.39 | |
| MgSO4 x 7 H2O | 6.3 | g | 6.3 | 25.56 | |
| MgCl2 x 6 H2O | 4.6 | g | 4.6 | 22.626 | |
| CaCl2 x 2 H2O | 1.3 | g | 1.3 | 8.843 | |
| NaHCO3 | 0.2 | g | 0.2 | 2.381 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water. | |||||
Metadata
| Last modified: | 08.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 24.7 | 422.656 |
| Agar | 15 | 44.599 |
| MgSO4 x 7 H2O | 6.3 | 25.56 |
| MgCl2 x 6 H2O | 4.6 | 22.626 |
| CaCl2 x 2 H2O | 1.3 | 8.843 |
| Show more | ||
