402 .
ALICYCLOBACILLUS MEDIUM
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* Medium for Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 454
Adjust pH of final medium to 3.0 - 4.0.
Growth conditions: 60 °C
Equipment needed:
Final pH:
* 3.0 - 4.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 1000 | ml | ||
|
|
Solution B | 1 | ml | ||
|
|
Solution C | 500 | ml | ||
| 1 Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l. | |||||
Solution A #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
(NH4)2SO4 | 0.20 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
Glucose | 5.00 | g | ||
|
|
KH2PO4 | 3.00 | g | ||
|
|
Distilled water
(for liquid medium) |
1000.00 | ml | ||
|
|
Distilled water
(for solid medium) |
500.00 | ml | ||
| 1 Adjust pH to 4.0 | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |