MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 402 . ALICYCLOBACILLUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 448

* Modification: Adjust pH of final medium to 3.0 - 4.0.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 60 °C

Main sol. 402 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1000 ml - -
Solution B 1 ml - -
Solution C 500 ml - -
1 Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.

Solution A #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.25 1.701
MgSO4 x 7 H2O 0.50 g 0.5 2.029
(NH4)2SO4 0.20 g 0.2 1.514
Yeast extract 2.00 g 2 -
Glucose 5.00 g 5 27.753
KH2PO4 3.00 g 3 22.045
Distilled water
(for liquid medium) 		1000.00 ml - -
Distilled water
(for solid medium) 		500.00 ml - -
1 Adjust pH to 4.0

Solution B #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-6 1 ml - -

Solution C #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
Agar 15 g 30 89.198
Distilled water 500 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 20.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 3.0 - 4.0
Edit in Medium builder

Associated strains

All strains for this medium: 39
Strains with modifications: 25
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 448

  3.0 - 4.0

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Glucose 5 27.753
KH2PO4 3 22.045
Yeast extract 2 -
MgSO4 x 7 H2O 0.5 2.029
Show more
CSV
JSON

Cultivation metadata from 39 strains BacDive

pH 4 - 6 4 strains
pH 2 - 4 3 strains
pH 6 - 8 2 strains
phyla 1 39 strains
oxygen aerobe 8 strains
Show more