Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Glucose | 5.00 | g | 5 | 27.753 | |
Mannitol | 5.00 | g | 5 | 27.447 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.406 | |
Na2MoO4 x 2 H2O | 5.00 | mg | 0.005 | 0.021 | |
K2HPO4 | 0.90 | g | 0.9 | 5.167 | |
KH2PO4 | 0.10 | g | 0.1 | 0.735 | |
FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
CaCO3 | 5.00 | g | 5 | 49.955 | |
Agar | 15.00 | g | 15 | 44.599 | |
Distilled water | 950.00 | ml | - | - | |
1 Adjust pH to 7.3. | |||||
2 Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.3 |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 40 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 15 | 44.599 |
CaCO3 | 5 | 49.955 |
Glucose | 5 | 27.753 |
Mannitol | 5 | 27.447 |
K2HPO4 | 0.9 | 5.167 |
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phyla | 1 | 40 strains |
temperature | mesophilic | 40 strains |