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DSMZ

DSMZ 390 . PYROBACULUM MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Pyrobaculum oguniense DSM 13380

* Modification: Omit Trypicase peptone. Increase amount of yeast extract to 1 g/l and lower amount of thiosulfate to 1 g/l. Adjust pH of final medium to 7.0.

Growth conditions: anaerobic 90 °C

Main sol. 390 #

ml
Equipment needed: Autoclave Filter Gassing station Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.287 9.741
KH2PO4 0.28 g 0.277 2.037
MgSO4 x 7 H2O 0.25 g 0.248 1.004
CaCl2 x 2 H2O 0.07 g 0.069 0.471
FeCl3 x 6 H2O 0.02 g 0.02 0.073
Allen's trace element solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Trypticase peptone* 0.50 g 0.495 -
Yeast extract* 1.00 g - -
Na2S2O3 x 5 H2O* 1.00 g - -
Na2S x 9 H2O 0.50 g 0.495 2.061
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except Trypticase peptone, yeast extract, sodium thiosulfate and sulfide), adjust pH to 6.0 and sparge medium with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add peptone, yeast extract, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation adjust pH of final medium to 6.0.

Allen's trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Last modified: 10.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: * 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 5
Strains with modifications: 4
Pyrobaculum oguniense DSM 13380

  1 g Na2S2O3 x 5 H2O
  1 g Yeast extract
  Na2S2O3 x 5 H2O
  Trypticase peptone
  Yeast extract
  7.0

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.287 9.741
Na2S2O3 x 5 H2O 0.99 3.989
Yeast extract 0.99 -
Na2S x 9 H2O 0.495 2.061
KH2PO4 0.277 2.037
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Cultivation metadata from 5 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 5 strains
nutrition autotroph 1 strain
oxygen anaerobe 4 strains
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