371
371 .
NATRONOBACTERIA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Natronococcus amylolyticus DSM 10524
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 371 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| KCl | 1.00 | g | 1 | 13.414 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgSO4 x 7 H2O | 0.24 | g | 0.24 | 0.974 | |
| CaSO4 x 2 H2O | 0.17 | g | 0.17 | 0.987 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Agar , if necessary | 20.00 | g | 20 | 59.465 | |
| NaCl | 200.00 | g | 200 | 3422.31 | |
| Na2-glutamate | 1.00 | g | 1 | 5.913 | |
|
Yeast extract
(Fisher BioReagents BP1422) |
5.00 | g | 5 | - | |
| Casamino acids | 5.00 | g | 5 | - | |
| Na2CO3 | 5.00 | g | 5 | 47.175 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add distilled water to give a final volume of 1000 ml. | |||||
| 2 Adjust pH to 6.5 before autoclaving! | |||||
| 3 Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5. | |||||
| 4 If agar medium is prepared, heat and dissolve agar before adding sodium chloride. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 - 9.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 20 |
| Strains with modifications: | 2 |
Natronococcus amylolyticus DSM 10524
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 200 | 3422.31 |
| Agar | 20 | 59.465 |
| Na2CO3 | 5 | 47.175 |
| Casamino acids | 5 | - |
| Yeast extract | 5 | - |
| Show more | ||
