358a
358a .
ACIDIANUS MEDIUM (ANAEROBIC)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Stygiolobus azoricus DSM 6296
* Modification: Reduce amount of yeast extract to 0.20 g/l and adjust pH to 2.5 - 3.0. Pressurize inoculated bottles to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
Growth conditions: anaerobic
80 °C
Main sol. 358a #
ml
Equipment needed: Serum bottle
Gassing station
Water bath
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.287 | 9.741 | |
| KH2PO4 | 0.28 | g | 0.277 | 2.037 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.248 | 1.004 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.069 | 0.471 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.073 | |
| Allen's trace element solution | 10.00 | ml | - | - | |
|
Sulfur
(powder) |
5.00 | g | 4.951 | 154.365 | |
|
Yeast extract*
(OXOID) |
0.20 | g | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients, except sulfur and yeast extract, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml medium in 100 ml serum bottles) containing already the appropriate amount of sulfur. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Add yeast extract from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere. | |||||
| 2 Pressurize inoculated bottles to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture. | |||||
| 3 Note: Inoculate with 5% (w/v) culture. Incubate without shaking. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MnCl2 x 4 H2O | 180 | mg | 0.18 | 0.91 | |
| Na2B4O7 x 10 H2O | 450 | mg | 0.45 | - | |
| ZnSO4 x 7 H2O | 22 | mg | 0.022 | 0.077 | |
| CuCl2 x 2 H2O | 5 | mg | 0.005 | 0.029 | |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 | |
| VOSO4 x 2 H2O | 3 | mg | 0.003 | 0.015 | |
| CoSO4 x 7 H2O | 1 | mg | 1.0e-3 | 0.006 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH of final solution to 2 with 1 N HCl. | |||||
Metadata
| Last modified: | 17.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | * 2.5 - 3.0 |
| Equipment needed: | Autoclave, Gassing station, Serum bottle, Water bath |
| Edit in Medium builder | |
Gas composition
- 80 % H2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 4.951 | 154.365 |
| (NH4)2SO4 | 1.287 | 9.741 |
| KH2PO4 | 0.277 | 2.037 |
| MgSO4 x 7 H2O | 0.248 | 1.004 |
| Yeast extract | 0.198 | - |
| Show more | ||
