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DSMZ

DSMZ 358a . ACIDIANUS MEDIUM (ANAEROBIC)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Acidianus ambivalens DSM 3772

* Modification: Reduce amount of yeast extract to 0.02 g/l.

Growth conditions: 2-3 days anaerobic 80 °C

Main sol. 358a #

ml
Equipment needed: Serum bottle Gassing station Water bath Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.30 g 1.287 9.741
KH2PO4 0.28 g 0.277 2.037
MgSO4 x 7 H2O 0.25 g 0.248 1.004
CaCl2 x 2 H2O 0.07 g 0.069 0.471
FeCl3 x 6 H2O 0.02 g 0.02 0.073
Allen's trace element solution 10.00 ml - -
Sulfur
(powder) 		5.00 g 4.951 154.365
Yeast extract*
(OXOID) 		0.02 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients, except sulfur and yeast extract, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 ml medium in 100 ml serum bottles) containing already the appropriate amount of sulfur. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Add yeast extract from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere.
2 Pressurize inoculated bottles to 1 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
3 Note: Inoculate with 5% (w/v) culture. Incubate without shaking.

Allen's trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.

Metadata

Last modified: 17.10.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 2.5
Equipment needed: Autoclave, Gassing station, Serum bottle, Water bath
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4
Strains with modifications: 3
Acidianus ambivalens DSM 3772

  Yeast extract
  0.02 g Yeast extract

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Sulfur 4.951 154.365
(NH4)2SO4 1.287 9.741
KH2PO4 0.277 2.037
MgSO4 x 7 H2O 0.248 1.004
CaCl2 x 2 H2O 0.069 0.471
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
oxygen aerobe 1 strain
temperature hyperthermophilic 3 strains
temperature thermophilic 1 strain