354
354 .
LÖWENSTEIN-JENSEN MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Mycobacterium sp. DSM 2966
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
28 °C
Main sol. 354 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 2.50 | g | 1.551 | 11.396 | |
| MgSO4 | 0.24 | g | 0.149 | 1.237 | |
| Mg-citrate | 0.60 | g | 0.372 | 1.736 | |
| L-Asparagin | 3.60 | g | 2.233 | 16.903 | |
| Potato flour | 30.00 | g | 18.61 | - | |
| Malachite green | 0.40 | g | 0.248 | 0.68 | |
| Distilled water | 600.00 | ml | - | - | |
| 1 Addition: | |||||
| Glycerol | 12.00 | ml | - | - | |
|
Fresh egg mixture
(yolk and whites, *) |
1000.00 | ml | - | - | |
| 2 pH 7.0 | |||||
| 3 Sterilize medium for 30 min at 121°C, cool to 30°C and add fresh egg mixture carefully to the medium. | |||||
| 4 * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 194 |
Mycobacterium sp. DSM 2966
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Potato flour | 18.61 | - |
| L-Asparagin | 2.233 | 16.903 |
| KH2PO4 | 1.551 | 11.396 |
| Mg-citrate | 0.372 | 1.736 |
| Malachite green | 0.248 | 0.68 |
| Show more | ||
Cultivation metadata from 194 strains
| pH | 6 - 8 | 2 strains |
| phyla | 2 | 200 strains |
| oxygen | obligate aerobe | 28 strains |
| oxygen | aerobe | 15 strains |
| oxygen | microaerophile | 8 strains |
| Show more | ||