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DSMZ

DSMZ 343 . THERMOTOGA NEAPOLITANA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 343 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Starch
(soluble) 		5.0 g 4.965 -
KH2PO4 0.5 g 0.497 3.649
Modified Wolin's mineral solution 15.0 ml - -
Artificial sea water 250.0 ml - -
Yeast extract
(OXOID) 		2.0 g 1.986 -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Wolin's vitamin solution (10x) 1.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.497 2.827
Na2S x 9 H2O 0.5 g 0.497 2.067
Distilled water 740.0 ml - -
1 Dissolve ingredients (except vitamins, sulfide, and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Dispense under the same gas atmosphere into anoxic Hungate-type tubes or serum vials to 30% of volume and autoclave. Add vitamins, sulfide, and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Sterilize vitamins by filtration. Adjust the pH of the complete medium to 6.5, if necessary.

Artificial sea water #

1000
Solution occurs in 4 other media (first defined in Medium 343).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 27.7 473.99
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
H3BO3 30.00 mg 0.03 0.485
SrCl2 x 6 H2O 15.00 mg 0.015 0.056
Citric acid 10.00 mg 0.01 0.052
KI 0.05 mg 5.0e-5 1.9e-4
CaCl2 x 2 H2O 2.25 g 2.25 15.305
Distilled water 1000.00 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 168 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 16.01.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 6.877 117.674
Starch 4.965 -
Yeast extract 1.986 -
MgSO4 x 7 H2O 1.738 7.051
MgCl2 x 6 H2O 1.365 6.716
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature hyperthermophilic 1 strain
temperature thermophilic 1 strain