340
340 .
CAPNOCYTOPHAGA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Capnocytophaga sputigena DSM 7273
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
anaerobic
37 °C
pH 7.00
Main sol. 340 #
ml
Equipment needed: Gassing station
Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
17 | g | 17 | - | |
| Yeast extract | 3 | g | 3 | - | |
| Glucose | 3 | g | 3 | 16.652 | |
| NaCl | 3 | g | 3 | 51.335 | |
| KNO3 | 3 | g | 3 | 29.673 | |
| Haemin | 3 | mg | 0.003 | 0.005 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Sterilize glucose separately. Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. | |||||
Metadata
| Last modified: | 07.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 90 % N2
- 10 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 4 |
Capnocytophaga sputigena DSM 7273
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase | 17 | - |
| NaCl | 3 | 51.335 |
| KNO3 | 3 | 29.673 |
| Glucose | 3 | 16.652 |
| Yeast extract | 3 | - |
| Show more | ||
