338
338 .
CAMPYLOBACTER RECTUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Campylobacter curvus DSM 6644
This strain uses the standard medium without modifications.
Growth conditions: 1-2 days
anaerobic
37 °C
pH 7.00
Main sol. 338 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Beef extract
(Difco) |
3.00 | g | 3 | - | |
|
Trypticase
(BBL) |
9.00 | g | 9 | - | |
| Yeast extract | 11.00 | g | 11 | - | |
| NaCl | 2.00 | g | 2 | 34.223 | |
| Na2HPO4 | 0.40 | g | 0.4 | 2.818 | |
| Na2CO3 | 0.25 | g | 0.25 | 2.359 | |
| Haemin | 5.00 | mg | 0.005 | 0.008 | |
| Na-formate | 2.00 | g | 2 | 29.409 | |
| Na-fumarate | 3.00 | g | 3 | 18.746 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2. | |||||
| 2 Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen. | |||||
Metadata
| Last modified: | 07.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Campylobacter curvus DSM 6644
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 11 | - |
| Trypticase | 9 | - |
| Na-fumarate | 3 | 18.746 |
| Beef extract | 3 | - |
| NaCl | 2 | 34.223 |
| Show more | ||
