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DSMZ

DSMZ 337 . SYNTROPHOCOCCUS SUCROMUTANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 337 #

ml
Equipment needed: Gassing station Hungate tubes Filter Autoclave Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 921 ml - -
Solution B 50 ml - -
Solution C 1 ml - -
Solution D 25 ml - -
Solution E 10 ml - -
Solution F 1 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 6.4 and dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions D, E and F are autoclaved separately under 100% N2 gas. To complete the medium add appropriate amounts of solutions B - F to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.4 - 6.8, if necessary.
2 Note: Rumen fluid may be replaced by supplementing the medium with 200 µg/ml of crude egg yolk phosphatidylcholine (SIGMA, type IX-E).

Solution A #

921
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral solution 50.0 ml - -
Trace element solution SL-10 1.0 ml - -
Clarified rumen fluid 300.0 ml - -
Trypticase peptone
(BD Bacto/BD BBL) 		5.0 g 5.429 -
Na-formate 0.6 g 0.651 9.579
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.4e-4 0.002
Distilled water 570.0 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.5 g 50 595.188
Distilled water 50.0 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Seven vitamins solution 1 ml - -

Solution D #

25
Compound Amount Unit Conc. [g/L] Conc. [mM]
Lactose 5 g 200 584.283
Distilled water 25 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.24 g 24 136.646
Distilled water 10.00 ml - -

Solution F #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 78 mg 78 324.754
Distilled water 1 ml - -

Mineral solution #

1000
Solution occurs in 4 other media (first defined in Medium 335).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 10.0 g 10 73.484
MgCl2 x 6 H2O 6.6 g 6.6 32.464
NaCl 8.0 g 8 136.893
NH4Cl 8.0 g 8 149.561
CaCl2 x 2 H2O 1.0 g 1 6.802
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 267 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Clarified rumen fluid #

1000
Solution occurs in 28 other media (first defined in Medium 1310).
1 Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min, dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then stored frozen at -20°C.

Seven vitamins solution #

1000
Solution occurs in 77 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 10.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.4 - 6.8
Equipment needed: Autoclave, Centrifuge, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Lactose 4.96 14.491
Trypticase peptone 4.96 -
NaHCO3 2.48 29.523
Na-formate 0.595 8.753
KH2PO4 0.496 3.645
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain