328
328 .
PYG MEDIUM WITH VOLATILE FATTY ACIDS
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Acetivibrio ethanolgignens DSM 3005
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 328 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Beef extract | 5.0 | g | 5 | - | |
|
Trypticase
(BBL) |
5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Mineral solution | 40.0 | ml | - | - | |
| (NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
| Fatty acid mixture | 3.1 | ml | - | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
|
Haemin
(dissolved in 0.5 ml 1 N NaOH) |
5.0 | mg | 0.005 | 0.008 | |
|
Vitamin K1
(0.5% sol. in ethanol) |
0.2 | ml | 1.0e-3 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Adjust pH to 6.9. | |||||
| 2 Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. | |||||
Solution occurs in 1
other media (first defined in Medium 328).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.30 | g | 0.3 | 2.041 |
| MgSO4 x 7 H2O | 0.48 | g | 0.48 | 1.947 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Acetic acid | 17 | ml | - | - |
| Propionic acid | 6 | ml | - | - |
| n-Butyric acid | 4 | ml | - | - |
| n-Valeric acid | 1 | ml | - | - |
| iso-Valeric acid | 1 | ml | - | - |
| iso-Butyric acid | 1 | ml | - | - |
| DL-2-Methylbutyric acid | 1 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.9 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 2 |
Acetivibrio ethanolgignens DSM 3005
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Yeast extract | 10 | - |
| Glucose | 5 | 27.753 |
| Beef extract | 5 | - |
| Trypticase | 5 | - |
| (NH4)2SO4 | 0.5 | 3.784 |
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