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31 .
ALKALINE NUTRIENT AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Alkalicoccobacillus gibsonii DSM 9728
* Modification: With 0.5% NaCl
Growth conditions: 30 °C
Main sol. 31 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , if necessary | 15 | g | 15 | 44.599 | |
| Distilled water | 900 | ml | - | - | |
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 | |||||
| Na-sesquicarbonate solution | 100 | ml | - | - | |
| NaCl* | 5 | g/l | - | - | |
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaHCO3 | 4.2 | g | 42 | 499.958 |
| Na2CO3 anhydrous | 5.3 | g | 53 | 500.057 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.7 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Na2CO3 anhydrous | 5.3 | 50.006 |
| NaCl | 5 | 85.558 |
| Peptone | 5 | - |
| NaHCO3 | 4.2 | 49.996 |
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