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DSMZ

DSMZ 315 . BACTEROIDES CELLULOSOLVENS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Pseudobacteroides cellulosolvens DSM 2933

This strain uses the standard medium without modifications.

Growth conditions: 35 °C

Main sol. 315 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.68 g 0.68 12.713
K2HPO4 0.30 g 0.3 1.722
KH2PO4 0.18 g 0.18 1.323
(NH4)2SO4 0.15 g 0.15 1.135
MgSO4 x 7 H2O 0.12 g 0.12 0.487
CaCl2 x 2 H2O 0.06 g 0.06 0.408
FeSO4 x 7 H2O 0.02 g 0.02 0.072
Trace element solution 10.00 ml - -
Vitamin solution 10.00 ml - -
Cellobiose OR Cellulose
(cellulose: i.e. MN 300) 		5.00 g 5 14.607
Resazurin 1.00 mg 1.0e-3 0.004
NaHCO3 2.00 g 2 23.808
L-Cysteine HCl x H2O 0.25 g 0.25 1.423
Na2S x 9 H2O 0.25 g 0.25 1.041
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2.
2 Filter sterilize cellobiose separately.

Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x H2O 0.500 g 0.5 2.958
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.020 g 0.02 0.042
H3BO3 0.010 g 0.01 0.162
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Na2SeO3 x 5 H2O 0.300 mg 3.0e-4 0.001
Distilled water 1000.000 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Vitamin solution #

1000
Solution occurs in 17 other media (first defined in Medium 315).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Pseudobacteroides cellulosolvens DSM 2933

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Cellulose 5 -
NaHCO3 2 23.808
NH4Cl 0.68 12.713
K2HPO4 0.3 1.722
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain