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DSMZ

DSMZ 311c . SPOROMUSA ACIDOVORANS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Pelosinus sp. DSM 26827

* Modification: Replace fructose with 2.50 g/l Na-(DL)-lactate added to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration.

Growth conditions: 2-3 days anaerobic 30 °C

Main sol. 311c #

ml
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.498 9.301
MgSO4 x 7 H2O 0.50 g 0.498 2.019
CaCl2 x 2 H2O 0.25 g 0.249 1.692
NaCl 2.25 g 2.239 38.31
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		2.00 ml 0.002 0.007
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Yeast extract 2.00 g 1.99 -
Casitone 2.00 g 1.99 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
K2HPO4 0.35 g 0.348 1.999
KH2PO4 0.23 g 0.229 1.682
Na2CO3 1.00 g 0.995 9.388
D-Fructose* 5.00 g 4.975 27.615
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O* 0.30 g 0.299 1.7
Na2S x 9 H2O* 0.30 g 0.299 1.243
DL-Dithiothreitol* 0.15 g - -
Na-(DL)-lactate* 2.50 g - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except phosphates, carbonate, fructose, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add phosphates, fructose, vitamins, cysteine and sulfide to the medium from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of fructose and vitamins should be sterilized by filtration. Adjust pH of complete medium to pH 6.5 - 7.0, if necessary.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 26.06.23
Source: DSMZ
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5 - 7.0
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 20
Strains with modifications: 12
Pelosinus sp. DSM 26827

  0.15 g DL-Dithiothreitol
  2.5 g Na-(DL)-lactate
  D-Fructose
  L-Cysteine HCl x H2O
  Na2S x 9 H2O

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na-(DL)-lactate 2.488 -
NaCl 2.239 38.31
Yeast extract 1.99 -
Casitone 1.99 -
Na2CO3 0.995 9.388
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Cultivation metadata from 20 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 20 strains
oxygen anaerobe 17 strains
oxygen obligate anaerobe 1 strain
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