Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
* Modification: Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas).
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
NH4Cl | 0.25 | g | 0.25 | 4.674 | |
NaCl | 1.00 | g | 1 | 17.112 | |
MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
KCl | 0.50 | g | 0.5 | 6.707 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
2,3-butanediol* | 0.90 | g | 0.9 | 9.987 | |
Na2S x 9 H2O | 0.36 | g | 0.36 | 1.499 | |
Triethanolamine hydrochloride* | 1.40 | g | - | - | |
Seven vitamins solution* | 1.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, 2,3-butanediol and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.2 - 7.5. | |||||
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 100 | mg | 0.1 | 0.074 |
p-Aminobenzoic acid | 80 | mg | 0.08 | 0.583 |
D-(+)-biotin | 20 | mg | 0.02 | 0.082 |
Nicotinic acid | 200 | mg | 0.2 | 1.625 |
Calcium pantothenate | 100 | mg | 0.1 | 0.21 |
Pyridoxine hydrochloride | 300 | mg | 0.3 | 1.459 |
Thiamine-HCl x 2 H2O | 200 | mg | 0.2 | 0.536 |
Distilled water | 1000 | ml | - | - |
Last modified: | 31.03.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.2 - 7.5 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Na2CO3 | 1.5 | 14.153 |
NaCl | 1 | 17.112 |
2,3-butanediol | 0.9 | 9.987 |
KCl | 0.5 | 6.707 |
MgCl2 x 6 H2O | 0.4 | 1.968 |
Show more |
phyla | 3 | 12 strains |
oxygen | anaerobe | 12 strains |
temperature | mesophilic | 12 strains |