25 .
RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM
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Equipment needed:
Autoclave
Tungsten lamp
Final pH:
4.9
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 0.25 | g | ||
|
|
Mannitol | 1.50 | g | ||
|
|
Na-gluconate | 0.50 | g | ||
|
|
KH2PO4 | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.40 | g | ||
|
|
NH4Cl | 0.40 | g | ||
|
|
NaCl | 0.40 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
Trace element solution SL-6 | 1.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 4.9, then add: | |||||
|
|
Fe(III) citrate
(0.1% in H2O) |
5.00 | ml | ||
|
|
Biotin
(0.002% in H2O) |
1.00 | ml | ||
|
|
p-Aminobenzoic acid
(0.01% in H2O) |
1.00 | ml | ||
|
|
Sodium thiosulfate pentahydrate
(10% in H2O) |
2.00 | ml | ||
| 2 Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |