248
248 .
OAT FLAKES AGAR WITH YEAST EXTRACT
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Actinobifida chromogena DSM 43367
This strain uses the standard medium without modifications.
Growth conditions: 50 °C
Main sol. 248 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Oat flakes | 20 | g | 20 | - | |
| Trace element solution | 1 | ml | - | - | |
| Yeast extract | 2 | g | 2 | - | |
| Agar | 20 | g | 20 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 19.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 41 |
Actinobifida chromogena DSM 43367
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | - |
| Oat flakes | 20 | - |
| Yeast extract | 2 | - |
| MnCl2 x 4 H2O | 1.0e-3 | 0.005 |
| FeSO4 x 7 H2O | 1.0e-3 | 0.004 |
| Show more | ||
Cultivation metadata from 41 strains
| pH | 6 - 8 | 3 strains |
| pH | 4 - 6 | 2 strains |
| pH | 8 - 10 | 2 strains |
| phyla | 3 | 41 strains |
| oxygen | aerobe | 12 strains |
| Show more | ||