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DSMZ

DSMZ 196 . DESULFOCAPSA SULFEXIGENS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 196 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 961 ml - -
Solution B 30 ml - -
Solution C 10 ml - -
1 First dissolve 2.7 g FeCl3 x 6 H2O in distilled water and adjust the pH to 7.0 with 1 N NaOH (about 3 ml). Add and dissolve remaining salts of solution A and sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 min, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is autoclaved separately under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B and C are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be adjusted to 7.2.
2 Note: For transfers use 5 - 10% (v/v) inoculum.

Solution A #

961
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 2.70 g 2.81 10.394
Na2SO4 3.00 g 3.122 21.978
KH2PO4 0.20 g 0.208 1.529
NH4Cl 0.30 g 0.312 5.836
NaCl 21.00 g 21.852 373.926
MgCl2 x 6 H2O 3.00 g 3.122 15.355
KCl 0.50 g 0.52 6.979
CaCl2 x 2 H2O 0.15 g 0.156 1.062
Trace element solution SL-10 1.00 ml - -
Distilled water 960.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

10
Equipment needed: Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2.5 g 250 1007.31
Distilled water 10.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 07.11.22
Source: DSMZ
Synonyms: DESULFOCAPSA SULFOEXIGENS MEDIUM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20.979 358.984
Na2SO4 2.997 21.1
MgCl2 x 6 H2O 2.997 14.742
FeCl3 x 6 H2O 2.697 9.979
Na2S2O3 x 5 H2O 2.498 10.063
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain