1885
1885 .
4X Plant Cell Medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Gamborg B5 Medium incl. Vitamins
(Duchefa G0210) |
3.163 | g | 3.163 | - | |
| 2,4-Dichlorophenoxyacetic Acid Stock Solution | 2.000 | ml | - | - | |
| 1-Naphtylacetic Acid Stock Solution | 0.500 | ml | - | - | |
| Indole-3-Acetic Acid | 0.500 | ml | - | - | |
| Kinetin Stock Solution | 0.400 | ml | - | - | |
|
Sucrose
(Duchefa S0809) |
20.000 | g | 20 | 58.428 | |
|
N-Z-Amine® A
(Sigma C7290) |
2.000 | g | 2 | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Adjust pH to 5.6. | |||||
| 3 For solid medium, use 8.0 g Plant Agar (Duchefa P1001) | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
Solution occurs in 4
other media (first defined in Medium 1881).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
2,4-Dichlorophenoxyacetic Acid
(Duchefa D0911) |
100 | mg | 1 | - | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
Solution occurs in 1
other media (first defined in Medium 1884).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
1-Naphtylacetic Acid
(Duchefa N0903) |
100 | mg | 1 | - | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 To prepare a 100 mg/ml stock solution, dissolve 100 mg of 1-Naphthylacetic Acid (NAA) in a few millilitres of 1 M NaOH, then fill up to 100 ml with absolute ethanol. | |||||
| 2 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Kinetin
(Duchefa K0905) |
50 | mg | 0.5 | 2.323 | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 To prepare a 0.5 mg/ml stock solution, dissolve 50 mg of Kinetin in a few millilitres of 1 M HCl, then fill up to 100 ml with absolute ethanol. | |||||
| 2 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
3-Indolyl acetic acid
(Duchefa I0901) |
100 | mg | 1 | 5.708 | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
Metadata
| Last modified: | 20.02.26 |
| Source: | MediaDive: DSMZ |
| Medium type: | Complex medium |
| Final pH: | 5.6 |
| Equipment needed: | Autoclave |
|
Description:
Medium for cultivation of PC 794, PC 1182, PC 1188 and PC 1189 plant cell lines. Medium modified after GAMBORG, O.L., R.A. MILLER and K. OJIMA: Exp. Cell Res., 50, 151-158, (1968) |
|
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