1884
1884 .
Linsmaier & Skoog LS Plant Cell Medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Linsmaier & Skoog Med. incl. vitamins
(Duchefa L0230) |
4.400 | g | 4.4 | - | |
| 2,4-Dichlorophenoxyacetic Acid Stock Solution | 0.220 | ml | - | - | |
| 1-Naphtylacetic Acid Stock Solution | 0.186 | ml | - | - | |
|
Sucrose
(Duchefa S0809) |
30.000 | g | 30 | 87.642 | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Adjust pH to 6.0. | |||||
| 3 For solid medium add Plant Agar as followed: | |||||
|
Plant Agar
(Duchefa P1001) |
9.000 | g | 9 | - | |
| 4 Autoclave at 121 °C for 20 min. | |||||
Solution occurs in 4
other media (first defined in Medium 1881).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
2,4-Dichlorophenoxyacetic Acid
(Duchefa D0911) |
100 | mg | 1 | - | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
Solution occurs in 1
other media (first defined in Medium 1884).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
1-Naphtylacetic Acid
(Duchefa N0903) |
100 | mg | 1 | - | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 To prepare a 100 mg/ml stock solution, dissolve 100 mg of 1-Naphthylacetic Acid (NAA) in a few millilitres of 1 M NaOH, then fill up to 100 ml with absolute ethanol. | |||||
| 2 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
Metadata
| Last modified: | 22.06.26 |
| Source: | MediaDive: DSMZ |
| Synonyms: | LS Plant Cell Medium × |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Autoclave |
|
Description:
Medium used for cultivation of PC 44, PC 273, PC 655 and PC 1017 plant cell lines. Modified after LINSMAIER, E.M. and F. SKOOG: Physiol. Plant., 18, 100-127, (1965) |
|
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