1881
1881 .
Gamborg B5 Medium including vitamins
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Gamborg B5 Medium incl. Vitamins
(Duchefa G0210) |
3.163 | g | 3.163 | - | |
| 2,4-Dichlorophenoxyacetic Acid Stock Solution | 2.000 | ml | - | - | |
|
Sucrose
(Duchefa S0809) |
20.000 | g | 20 | 58.428 | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Adjust pH to 5.5. | |||||
| 3 For solid medium, use 8.0 g Plant Agar (Duchefa P1001). | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
Solution occurs in 4
other media (first defined in Medium 1881).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
2,4-Dichlorophenoxyacetic Acid
(Duchefa D0911) |
100 | mg | 1 | - | |
| Ethanol absolute | 100 | ml | - | - | |
| 1 Store in a glass bottle with a ground glass stopper at 4 °C. | |||||
Metadata
| Last modified: | 20.02.26 |
| Source: | MediaDive: DSMZ |
| Synonyms: | B5 Plant cell medium × |
| Medium type: | Complex medium |
| Final pH: | 5.5 |
| Equipment needed: | Autoclave |
|
Description:
Medium for plant cell line cultivation. Medium modified after GAMBORG, O.L., R.A. MILLER and K. OJIMA: Exp. Cell Res., 50, 151-158, (1968). Medium 1881 is used for cultivating PC 1026 (Glycine max) and PC 998 (Triticum aestivum) cell lines. |
|
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