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DSMZ

DSMZ 1875 . Medium MYGS

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1875 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
MES [2-(N-morpholino) ethane sulfonic acid] 1.95 g 1.95 9.988
Trace element solution SL-10 2.00 ml - -
Selenite-tungstate solution 2.00 ml - -
20 mM MgSO4 x 7 H2O 20.00 ml - -
30 mM CaCl2 x 2 H2O 20.00 ml - -
20mM (NH4)2HPO4 20.00 ml - -
Yeast extract 0.50 g 0.5 -
Vitamin solution 3.00 ml - -
Starch 0.40 g 0.4 -
D(+)-Glucose 0.45 g 0.45 2.498
Glycerol 0.70 ml - -
Agar 20.00 g 20 -
Distilled water 940.00 ml - -
1 Adjust pH to 7.2.
2 Agar und die restlichen Bestandteile getrennt autoklavieren (20 g Agar in 500 ml dest. H2O + Rest der Zutaten in 440 ml dest. H2O)
3 Vitaminlösung erst nach dem Autoklavieren dazugeben

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

20 mM MgSO4 x 7 H2O #

100
Solution occurs in 2 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
MgSO4 x 7 H2O 0.49 g 4.9 19.88

30 mM CaCl2 x 2 H2O #

100
Solution occurs in 2 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
CaCl2 x 2 H2O 0.44 g 4.4 29.929

20mM (NH4)2HPO4 #

100
Solution occurs in 2 other media (first defined in Medium 1266).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H2O 100.00 ml - -
(NH4)2HPO4 0.26 g 2.6 19.688

Vitamin solution #

1000
Solution occurs in 3 other media (first defined in Medium 1266).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 17 mg 0.017 0.013
4-aminobenzoate 13 mg 0.013 0.095
Biotin 3 mg 0.003 0.012
Nicotinic acid 33 mg 0.033 0.268
Hemicalcium D-(+)-pantothenate 17 mg 0.017 -
Pyridoxamine hydrochloride 50 mg 0.05 0.244
Thiamine-HCl x 2 H2O 33 mg 0.033 0.088
DL-6,8-thioctic acid 10 mg 0.01 0.048
Riboflavin 10 mg 0.01 0.027
Folic acid 4 mg 0.004 0.009
Distilled water 1000 ml - -
1 Filter sterilize the solution.

Metadata

Last modified: 30.10.25
Source: MediaDive: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 -
MES [2-(N-morpholino) ethane sulfonic acid] 1.95 9.988
Yeast extract 0.5 -
D(+)-Glucose 0.45 2.498
Starch 0.4 -
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