DSMZ 1848 . Rhizobium medium for isolation

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
D-Mannitol 10.0 g 10 54.894
K2HPO4 0.5 g 0.5 2.871
MgSO4 0.2 g 0.2 1.662
Yeast extract 1.0 g 1 -
NaCl 0.1 g 0.1 1.711
Agar 20.0 g 20 -
1 Make up to 1000 ml with distilled water.
2 Heat to boiling to dissolve the medium completely
3 Autoclave at 121 °C for 15 min.
4 Cool down to 45-50°C.

Metadata

Last modified: 10.10.24
Source: MediaDive: DSMZ
Synonyms: Rhizobium medium
Medium type: Complex medium
Final pH: 6.6 - 7.0
Equipment needed: Autoclave
Description:

Available as M408 HIMEDIA

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Associated strains

All strains for this medium: 5

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 20 -
D-Mannitol 10 54.894
Yeast extract 1 -
K2HPO4 0.5 2.871
MgSO4 0.2 1.662
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Cultivation metadata from 5 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 5 strains
oxygen aerobe 3 strains
oxygen obligate aerobe 2 strains
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