1831a
1831a .
1/2 SWES - Brackish Water Medium
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1831a #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Natural sea water | 455.0 | ml | - | - | |
|
KNO3
(19 g/l stock solution) |
10.5 | ml | - | - | |
|
K2HPO4
(4.35 g/l stock solution) |
4.6 | ml | - | - | |
|
MgSO4 x 7 H2O
(7.5 g/l stock solution) |
2.7 | ml | - | - | |
| Micronutrient Solution * | 5.0 | ml | - | - | |
| Soil Extract ** | 30.0 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 Add aseptically after autoclaving: | |||||
|
Vitamin B12
(0.02 g/l stock solution, filter sterilised) |
250.0 | μl | - | - | |
Solution occurs in 1
other media (first defined in Medium 1831).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Milli Q Water | 700.0 | ml | - | - | |
|
Fe SO4 x 7 H2O
(10.0 g/l stock solution) |
70.0 | ml | - | - | |
|
Na-EDTA x 2 H2O
(80.0 g/l stock solution) |
10.0 | ml | - | - | |
|
ZnSO4 x 7 H2O
(1.0 g/l stock solution) |
1.0 | ml | - | - | |
|
MnSO4 x 1 H2O
(0.76 g/l stock solution) |
2.0 | ml | - | - | |
|
H3BO3
(2.0 g/l stock solution) |
5.0 | ml | - | - | |
|
Co(NO3)2 x 6 H2O
(5.0 g/l stock solution) |
0.2 | ml | - | - | |
|
Na2 MoO4 x 2 H2O
(0.2 g/l stock solution) |
5.0 | ml | - | - | |
|
CuSO4 x 5 H2O
(0.25 g/l stock solution) |
20.0 | μl | - | - | |
| 1 Make up to 1000 ml with Milli Q water. | |||||
| 2 Prepare aliquots and store them at -20 °C. | |||||
Solution occurs in 1
other media (first defined in Medium 1831).
Equipment needed: Autoclave
| 1 Preparation of soil extract: Fill a 1 litre Schott bottle one third with garden or leaf soil of medium, but not too high humus content. The soil must not contain fertilizers or plant protective agents. Success of soil extract depends on selection of suitable soils. Those with high clay content are usually less satisfactory. Make up with Milli Q water to 700 ml and sterilize by autoclaving for one hour twice in a 24 h interval. Separate the decanted extract from particles by centrifugation. Filter the supernatant through Whatman filter paper into a 1 litre Schott bottle, autoclave for 20 min at 121°C and store in the refrigerator. To prepare the medium, aseptically remove 30 ml of the soil extract. |
Metadata
| Last modified: | 21.02.24 |
| Source: | MediaDive: DSMZ |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
|
Description:
Medium for the cultivation of brackish cyanobacteria and protists |
|
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 8 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Natural sea water | - | - |
| K2HPO4 | - | - |
| MgSO4 x 7 H2O | - | - |
| ZnSO4 x 7 H2O | - | - |
| Na-EDTA x 2 H2O | - | - |
| Show more | ||
Cultivation metadata from 8 strains
| phyla | 1 | 7 strains |