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SELENOMONAS RUMINANTIUM MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Selenomonas sp. DSM 2924
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Main sol. 181 #
ml
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 1.0 | g | 1 | 5.551 | |
|
Trypticase
(BBL) |
5.0 | g | 5 | - | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| Na-acetate | 4.0 | g | 4 | 48.76 | |
| Yeast extract | 2.0 | g | 2 | - | |
| n-Valeric acid | 0.1 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Na2CO3 | 4.0 | g | 4 | 37.74 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0. Gas atmosphere: 100% CO2. | |||||
| 2 Distribute into Hungate tubes and autoclave. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Selenomonas sp. DSM 2924
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase | 5 | - |
| Na-acetate | 4 | 48.76 |
| Na2CO3 | 4 | 37.74 |
| Yeast extract | 2 | - |
| KH2PO4 | 1 | 7.348 |
| Show more | ||
