MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 1801 . SULFURIMONAS MARISNIGRI MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1801 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1001 ml - -
Solution B 15 ml - -
Solution C 2 ml - -
Solution D 2 ml - -
Solution E 2 ml - -
Solution F 3 ml - -
Solution G 1 ml - -
1 Dissolve ingredients of solution A (except MnO2), then sparge with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute solution A under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing the appropriate amount of MnO2 and autoclave at 121°C for 15 min. Solution B is autoclaved separately under an 80% N2 and 20% CO2 gas atmosphere, and solutions C, D, and E are autoclaved under a 100% N2 gas atmosphere. Solutions F and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. The final pH of the complete medium should be 7.5.

Solution A #

1001
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 17.7 g 17.682 302.572
MgCl2 x 6 H2O 3.2 g 3.197 15.724
CaCl2 x 2 H2O 0.4 g 0.4 2.718
KCl 0.7 g 0.699 9.38
MgSO4 x H2O 2.7 g 2.697 22.409
Trace element solution SL-10 1.0 ml - -
MnO2 0.5 g 0.5 5.746
Distilled water 1000.0 ml - -

Solution B #

15
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 0.75 g 50 471.752
Distilled water 15.00 ml - -

Solution C #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.01 g 5 93.475
Distilled water 2.00 ml - -

Solution D #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.01 g 5 28.707
Distilled water 2.00 ml - -

Solution E #

2
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-acetate 0.16 g 80 975.208
Distilled water 2.00 ml - -

Solution F #

3
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 0.75 g 250 1007.31
Distilled water 3.00 ml - -

Solution G #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 308 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 12.01.23
Source: MediaDive: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5
Edit in Medium builder

Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 17.251 295.199
MgCl2 x 6 H2O 3.119 15.341
MgSO4 x H2O 2.632 21.863
Na2CO3 0.731 6.897
Na2S2O3 x 5 H2O 0.731 2.945
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain