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DSMZ

DSMZ 1748 . HALOFERAX GIBBONSII MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Haloferax gibbonsii DSM 112399

This strain uses the standard medium without modifications.

Growth conditions: 2-3 days aerobic 37 °C

Main sol. 1748 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mineral Salts Solution for solid 20%MGM 1000 ml - -
1 or
Mineral Salts Solution for liquid 23% MGM 1000 ml - -

Mineral Salts Solution for liquid 23% MGM #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Salt Water Solution (30% Stock Solution) 786 ml - -
Peptone
(Oxoid) 		5 g 5 -
Yeast extract
(Fisher) 		1 g 1 -
Tap water 1000 ml - -
Agar , for solid medium 20 g 20 59.465
1 Adjust to pH 7.2 with 1 M Tris-Base and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.

Mineral Salts Solution for solid 20%MGM #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Salt Water Solution (30% Stock Solution) 666 ml - -
Peptone
(Oxoid) 		5 g 5 -
Yeast extract
(Fisher) 		1 g 1 -
Tap water 1000 ml - -
Agar , for solid medium 20 g 20 59.465
1 Adjust to pH 7.2 with 1 M Tris-Base and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.

Salt Water Solution (30% Stock Solution) #

1000
Solution occurs in 2 other media (first defined in Medium 1339).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 240 g 240 4106.78
KCl 7 g 7 93.897
MgCl2 x 6 H2O 30 g 30 147.563
MgSO4 x 7 H2O 35 g 35 142.002
CaCl2
(1 M) 		5 ml 0.555 5
Distilled water 995 ml - -
1 Adjust to pH 7.5 with 2 M Tris-Base and sterilize by autoclaving.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 2
Haloferax gibbonsii DSM 112399

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 187.702 3211.86
MgSO4 x 7 H2O 27.373 111.058
MgCl2 x 6 H2O 23.463 115.408
Agar 20 59.465
KCl 5.475 73.436
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Cultivation metadata from 2 strains BacDive

phyla 1 1 strain