174
174 .
ZUELZERA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 174 #
ml
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| D-Glucose | 5.0 | g | 5 | 27.753 | |
|
Yeast extract
(OXOID) |
2.0 | g | 2 | - | |
|
Peptone
(BD BACTO) |
2.0 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na-thioglycolate | 0.5 | g | 0.5 | 4.382 | |
|
Agar
, for stabs
optional |
10.0 | g | 10 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients, adjust pH to 7.6 with KOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
| 2 For stabs add 10.0 g/l agar. | |||||
Metadata
| Last modified: | 25.10.22 |
| Source: | DSMZ |
| Synonyms: | TREPONEMA STENOSTREPTUM MEDIUM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.6 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
|
Agar optional |
10 | - |
| D-Glucose | 5 | 27.753 |
| Peptone | 2 | - |
| Yeast extract | 2 | - |
| Na-thioglycolate | 0.5 | 4.382 |
| Show more | ||
