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DSMZ

DSMZ 1738 . METHYLOMONAS MEDIUM (WSC)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Methylomonas rosea DSM 112281

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days microaerobic 20 °C

Main sol. 1738 #

ml
1 The growth medium contains the following components (per liter):
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (20X Basal Salts Solution) 50.0 mL - -
Solution 2 (Wolfe's Vitamin Solution) 10.0 mL - -
Solution 3 (Acidic Trace Elements Solution) 0.5 mL - -
Solution 4 (Alkaline Trace Elements Solution) 0.2 mL - -
Solution 5 (40X Buffer Solution) 25.0 mL - -
Distilled water 914.3 ml - -
2 Combine the distilled water and Solutions 1,3,4 in an Erlenmeyer flask and dispense into glass serum bottles.
3 Flush the headspace of the serum bottles with pure N2. Seal the serum bottles with butyl rubber stoppers and crimp seals. Using a syringe and needle, inject pure methane and oxygen to final headspace concentrations of 5-10% CH4 and 21% O2.
4 Autoclave the serum bottles for 20 minutes at 121 °C.
5 Upon cooling to room temperature, add Solutions 2 and 5 from a filter-sterilized stock solution.
6 The final pH of the medium should be 7.8 - 7.9.

Solution 1 (20X Basal Salts Solution) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 5.26 g 5.26 90.007
KCl 2.24 g 2.24 30.047
MgSO4 x 7 H2O 2.46 g 2.46 9.981
NaNO3 0.85 g 0.85 10.001
CaCl2 x 2 H2O 0.88 g 0.88 5.986
H3BO3 0.08 g 0.08 1.294
SrCl2 x 6 H2O 0.14 g 0.14 0.525
1 Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre

Solution 2 (Wolfe's Vitamin Solution) #

1000
Solution occurs in 1 other media (first defined in Medium 1738).
Compound Amount Unit Conc. [g/L] Conc. [mM]
4-Aminobenzoic Acid 5.0 mg 0.005 0.036
D-Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Riboflavin 5.0 mg 0.005 0.013
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-Pantothenate 5.0 mg 0.005 -
Thioctic acid
(Lipoic Acid) 		5.0 mg 0.005 0.024
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Distilled water 1000.0 ml - -

Solution 3 (Acidic Trace Elements Solution) #

1000
Solution occurs in 1 other media (first defined in Medium 1738).
1 Contains the following components per liter of deionized water:
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(5M) 		20.00 mL 3.646 100
FeSO4 x 7 H2O 2.09 g 2.09 7.518
ZnSO4 x 7 H2O 0.07 g 0.07 0.243
CoCl2 0.06 g 0.06 0.462
MnCl2 x 4 H2O 0.50 g 0.5 2.526
CuSO4 x 5 H2O 0.50 g 0.5 2.003
NiCl2 x 6 H2O 0.10 g 0.1 0.772
H3BO3 0.01 g 0.01 0.162
La(NO3)3 x 6 H2O 0.06 g 0.06 -
Ce(NO3)3 x 6 H2O 0.06 g 0.06 -
Distilled water 1000.00 ml - -

Solution 4 (Alkaline Trace Elements Solution) #

1000
Solution occurs in 1 other media (first defined in Medium 1738).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.40 g 0.4 10.001
Na2SeO4 0.11 g 0.11 0.582
Na2WO4 x 2 H2O 0.05 g 0.05 0.152
Na2MoO4 x 2 H2O 0.29 g 0.29 1.199
Distilled water 1000.00 ml - -

Solution 5 (40X Buffer Solution) #

1000
Solution occurs in 1 other media (first defined in Medium 1738).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HEPES
(free acid) 		95.30 g 95.3 399.916
NaOH 11.80 g 11.8 295.022
Na2HPO4 5.68 g 5.68 40.012
Distilled water 1000.00 ml - -
1 Adjust pH 7.8-7.9

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.8 - 7.9
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Associated strains

All strains for this medium: 2
Methylomonas rosea DSM 112281

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
HEPES 2.383 9.998
NaOH 0.295 7.376
NaCl 0.263 4.5
Na2HPO4 0.142 1
MgSO4 x 7 H2O 0.123 0.499
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains