1728
1728 .
MMB MEDIUM (ATCC 2254)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1728 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.1 | g | 0.1 | - | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Glucose | 1.0 | g | 1 | 5.551 | |
| (NH4)2SO4 | 0.2 | g | 0.2 | 1.514 | |
| Hutner's salts | 20.0 | ml | - | - | |
| Vitamin solution | 10.0 | ml | - | - | |
| Distilled water | 970.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | 44.599 | |
| 1 pH 7.0- 7.5 | |||||
| 2 For solid medium, add 15g of agar per liter. | |||||
| 3 Glucose and vitamin solution should be filter-sterilized separately. | |||||
Solution occurs in 24
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | 0.007 | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.000 | ml | - | - | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid. | |||||
| 2 Adjust pH to 7.0 with KOH (~7.3 g). | |||||
| 3 Dissolve other salts separately and combine. | |||||
| 4 Adjust pH to 6.8 with NaOH or H2SO4. | |||||
Solution occurs in 34
other media (first defined in Medium 590).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na-EDTA | 250.0 | mg | 0.25 | 0.744 | |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 | |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 | |
| MnSO4 x H2O | 154.0 | mg | 0.154 | 0.911 | |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 | |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 | |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| D-Calcium pantothenate | 5.0 | mg | 0.005 | - |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 - 7.5 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Glucose | 1 | 5.551 |
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| (NH4)2SO4 | 0.2 | 1.514 |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Show more | ||
