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DSMZ

DSMZ 1721 . MMJHS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Nitratiruptor labii DSM 111345

* Modification: Adjust pH of final medium to 6.0.

Growth conditions: 1-2 days facultative anaerobic 55 °C

Main sol. 1721 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 1 g 1 11.765
NaHCO3 1 g 1 11.904
Na2S2O3 x 5 H2O 1 g 1 4.029
Sulfur
(powdered) 		10 g 10 311.818
Synthetic seawater
(MJ) 		1000 ml - -
1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding NaNO3, Na2S2O3 x 5 H20 and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, NaNO3, Na2S2O3 x 5 H20 and NaHCO3.
2 Increase the 80% H2 + 20% CO2 gas phase pressure to 0.3 MPa.
3 Adjust pH strain-specifically.

Synthetic seawater #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 25 427.789
K2HPO4 0.14 g 0.14 0.804
CaCl2 x 2 H2O 0.70 g 0.7 4.761
NH4Cl 0.25 g 0.25 4.674
MgSO4 x 7 H2O 3.40 g 3.4 13.794
MgCl2 x 6 H2O 4.20 g 4.2 20.659
KCl 0.50 g 0.5 6.707
Trace mineral solution 10.00 ml - -
Distilled water 1000.00 ml - -

Trace mineral solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.500 g 1.5 7.848
MgSO4 x 7 H2O 3.000 g 3 12.172
MnSO4 x n H2O 0.500 g 0.5 -
NaCl 1.000 g 1 17.112
FeSO4 x 7 H2O 0.100 g 0.1 0.36
CoSO4 x 7 H2O 0.180 g 0.18 1.161
CaCl2 x 2 H2O 0.100 g 0.1 0.68
ZnSO4 x 7 H2O 0.180 g 0.18 0.626
CuSO4 x 5 H2O 0.010 g 0.01 0.04
AlK(SO4)2 2.000 mg 0.002 0.008
H3BO3 0.010 g 0.01 0.162
Na2WO4 x 2 H2O 0.100 g 0.1 0.303
NiCl2 x 6 H2O 0.025 g 0.025 0.193
Distilled water 1000.000 ml - -
Na2MoO4 x 2 H2O 0.010 g 0.01 0.041
Na2SeO3 x 5 H2O 0.050 g 0.05 0.19
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Metadata

Last modified: 05.06.24
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: * 6.0
Equipment needed: Filter
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Gas composition

  • 80 % H2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Strains with modifications: 2
Nitratiruptor labii DSM 111345

  6.0

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 10 311.818
MgCl2 x 6 H2O 4.2 20.659
NaHCO3 1 11.904
NaNO3 1 11.765
Na2S2O3 x 5 H2O 1 4.029
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
temperature thermophilic 2 strains