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DSMZ

DSMZ 1714 . CONEXIVISPHAERA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1714 #

ml
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.20 g 0.198 1.497
KH2PO4 3.00 g 2.967 21.805
Allen's trace element solution 10.00 ml - -
Na2S2O3 x 5 H2O 2.50 g 2.473 9.963
MgSO4 x 7 H2O 0.50 g 0.495 2.007
CaCl2 x 2 H2O 0.25 g 0.247 1.682
Yeast extract
(OXOID) 		0.50 g 0.495 -
Wolin's vitamin solution (10x) 1.00 ml - -
L-Cysteine HCl x H2O 0.05 g 0.049 0.282
FeCl2 x 4 H2O 0.25 g 0.247 1.244
Distilled water 1000.00 ml - -
1 Dissolve ammonium sulfate, potassium phosphate and trace elements, adjust pH to 5.2 using 10 N H2SO4 and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium sulfate, calcium chloride, yeast extract, vitamins (sterilized by filtration), cysteine and ferrous chloride from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 5.0 - 5.2, if necessary.

Allen's trace element solution #

1000
Solution occurs in 14 other media (first defined in Medium 88).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnCl2 x 4 H2O 180 mg 0.18 0.91
Na2B4O7 x 10 H2O 450 mg 0.45 -
ZnSO4 x 7 H2O 22 mg 0.022 0.077
CuCl2 x 2 H2O 5 mg 0.005 0.029
Na2MoO4 x 2 H2O 3 mg 0.003 0.012
VOSO4 x 2 H2O 3 mg 0.003 0.015
CoSO4 x 7 H2O 1 mg 1.0e-3 0.006
Distilled water 1000 ml - -
1 Adjust pH of final solution to 2 with 1 N HCl.

Wolin's vitamin solution (10x) #

1000
Solution occurs in 305 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 5.0 - 5.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 2.967 21.805
Na2S2O3 x 5 H2O 2.473 9.963
MgSO4 x 7 H2O 0.495 2.007
Yeast extract 0.495 -
CaCl2 x 2 H2O 0.247 1.682
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain