Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Solution A | 994.0 | ml | ||
|
Solution B | 0.1 | ml | ||
|
Solution C | 0.1 | ml | ||
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and C are autoclaved separately under 100% N2 gas. Adjust pH of complete medium to 7.2 - 7.4, if necessary. | |||||
2 Use 1.10 g/l Na-pyruvate as substrate. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. |
Compound | Amount | Unit | |
---|---|---|---|
|
KH2PO4 | 0.020 | g |
|
NH4Cl | 0.025 | g |
|
NaCl | 0.100 | g |
|
MgCl2 x 6 H2O | 0.040 | g |
|
KCl | 0.050 | g |
|
CaCl2 x 2 H2O | 0.015 | g |
|
NaNO3 | 0.850 | g |
|
Yeast extract | 0.100 | g |
|
Sodium resazurin
(0.1% w/v) |
0.050 | ml |
|
Distilled water | 994.000 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | ||
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
ZnCl2 | 70.0 | mg | ||
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
H3BO3 | 6.0 | mg | ||
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
Distilled water | 990.0 | ml | ||
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Compound | Amount | Unit | |
---|---|---|---|
|
NaOH | 0.5 | g |
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
Distilled water | 1000.0 | ml |