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DSMZ

DSMZ 1687 . THERMOANAEROBACULUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1687 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 994.0 ml - -
Solution B 0.1 ml - -
Solution C 0.1 ml - -
1 Sparge solution A with 100% N2 gas for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and C are autoclaved separately under 100% N2 gas. Adjust pH of complete medium to 7.2 - 7.4, if necessary.
2 Use 1.10 g/l Na-pyruvate as substrate. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration.

Solution A #

994
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.020 g 0.02 0.148
NH4Cl 0.025 g 0.025 0.47
NaCl 0.100 g 0.101 1.721
MgCl2 x 6 H2O 0.040 g 0.04 0.198
KCl 0.050 g 0.05 0.675
CaCl2 x 2 H2O 0.015 g 0.015 0.103
NaNO3 0.850 g 0.855 10.061
Yeast extract 0.100 g 0.101 -
Sodium resazurin
(0.1% w/v) 		0.050 ml 5.0e-5 -
Distilled water 994.000 ml - -

Solution B #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -

Solution C #

1
Equipment needed: Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Selenite-tungstate solution 1 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 267 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 180 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaNO3 0.855 10.061
NaCl 0.101 1.721
Yeast extract 0.101 -
KCl 0.05 0.675
MgCl2 x 6 H2O 0.04 0.198
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature thermophilic 1 strain