1686
1686 .
Medium for Lichenibacterium
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1686 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose OR Mannitol | 1.00 | g | 1 | 5.551 | |
| NH4NO3 | 0.20 | g | 0.2 | - | |
| NaCl | 0.20 | g | 0.2 | 3.422 | |
| KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
| MgSO4 | 0.04 | g | 0.04 | 0.332 | |
| Ca(NO3)2 x 4 H2O | 0.04 | g | 0.04 | 0.169 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| Vitamin solution | 0.50 | ml | - | - | |
| 1 Autoclave at 121°C for 15 min. | |||||
| 2 After sterilization add 0,5 ml/l filter-sterilized vitamin solution (see Medium 462). | |||||
| 3 Adjust pH to 5 - 6. | |||||
Solution occurs in 4
other media (first defined in Medium 462).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| p-Aminobenzoate | 1.0 | mg | 0.01 | 0.073 |
| Biotin | 0.2 | mg | 0.002 | 0.008 |
| Nicotinic acid | 2.0 | mg | 0.02 | 0.162 |
| Thiamine-HCl x 2 H2O | 1.0 | mg | 0.01 | 0.027 |
| Calcium pantothenate | 0.5 | mg | 0.005 | 0.01 |
| Pyridoxamine | 5.0 | mg | 0.05 | 0.297 |
| Vitamin B12 | 2.0 | mg | 0.02 | 0.015 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 5.0 - 6.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |